Lacto-N-neotetraose (LNnT) is a tetrasaccharide formed of N‑acetyllactosamine linked with lactose. LNnT belongs to the neutral non-fucosylated human milk oligosaccharide category (Kunz et al. 2000). It is one of the most abundant oligosaccharides in human milk with reported means between 0.08 ‑ 0.20 g/l (Urashima et al. 2012). In combination with 2'FL, LNnT is approved for addition to infant formulas in the European Union since 2016 (EU/2016/375).